For each sequencing library, for each annotation family, ncPRO-seq generates two compressed track files in http://genome.ucsc.edu/goldenPath/help/bedgraph.html BedGraph format to describe read mapping in sense and antisense direction of annotation items respectively. These track files can be then, easily upload in a visualisation tool as the UCSC Genome Browser [4].
Moreover, the interface allows users to select different subsets of reads mapped in the genome to generate BedGraph formatted track files.
The minimum and maximum size of the reads, as well as the minimum and maximum number of locations can be used to filter the aligned reads (Figure 5).
Figure 5:
ncPRO-seq web interface : genome tracks options
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Chongjian Chen
2012-01-26
