Alignment pre-processing

ncPRO-seq can also take read alignment files in BAM format as input. BAM is the compressed binary version of the SAM format, please check http://samtools.sourceforge.net/ here for more information. The GROUP_READ option also works for BAM file input. The clustering process is based on read sequences in alignments using the similar method as described in 5.2.1. After this step, a new bam file will be generated by replacing original read information with read group and removing redundant alignments in original bam file.



Nicolas Servant 2012-05-31